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| Rekha,Rani; Alam Rizvi,Moshahid; Jaishree,Paul. |
| The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual. |
| Tipo: Journal article |
Palavras-chave: 16S rRNA based primers; Anaerobic bacteria; Diarrhoea patient; Polymerase chain reaction. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500005 |
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| Silva,Clara Maria Guimarães; Silva,Déborah Nascimento dos Santos; Costa,Scarlathe Bezerra da; Almeida,Juliana Soares de Sá; Boente,Renata Ferreira; Teixeira,Felipe Lopes; Domingues,Regina Maria Cavalcanti Pilotto; Lobo,Leandro Araujo. |
| ABSTRACT Bacteroides fragilis is the strict anaerobic bacteria most commonly found in human infections, and has a high mortality rate. Among other virulence factors, the remarkable ability to acquire resistance to a variety of antimicrobial agents and to tolerate nanomolar concentrations of oxygen explains in part their success in causing infection and colonizing the mucosa. Much attention has been given to genes related to multiple drug resistance derived from plasmids, integrons or transposon, but such genes are also detected in chromosomal systems, like the mar (multiple antibiotic resistance) locus, that confer resistance to a range of drugs. Regulators like MarR, that control expression of the locus mar, also regulate resistance to organic solvents,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacteroides fragilis; Anaerobic bacteria; Multi-drug resistance; Oxidative stress resistance. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100200 |
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| Yehya,Mariam; Hamze,Monzer; Mallat,Hassan; Dabbousi,Fouad. |
| Fifty one strains of the Bacteroides fragilis group were isolated from 45 fecal samples. Classical phenotypic identification showed that 16 isolates were B. thetaiotaomicron, 12 B. uniformis, 9 B. eggerthii,7 B. vulgatus,3 B. caccae,2 Parabacteroides distasonis with 1 identified B. ovatus and 1 B. fragilis. The 51 strains were tested for susceptibility against 16 antimicrobial agents and the MICs for metronidazole were determined. The tests showed that imipenem, meropenem and chloram-phenicol were the most effective antibiotics (98%, 98% and 92.16% of susceptibility, respectively) followed by ticarcillin/clavulanic acid, piperacillin/tazobactam, rifampin (88.24% susceptibility), moxifloxacin 86.27% and tigecycline 84.31%. Ofloxacin and cefotaxime were the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacteroides fragilis group; Anaerobic bacteria; Antibiotics susceptibility. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300022 |
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